PrimePCR™ Probe Assay: EDARADD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EDARADD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EDARADD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039636
Assay Design:   Intron-spanning
Chromosome Location:   1:236577560-236631539question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000439430 ENST00000334232 ENST00000359362

Gene Information

This gene was identified by its association with ectodermal dysplasia a genetic disorder characterized by defective development of hair teeth and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein and is found to interact with EDAR a death domain receptor known to be required for the development of hair teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR this protein acts as an adaptor and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   EDARADD
Gene Name:   EDAR-associated death domain
Aliases:   ED3, EDA3
RefSeq:   NC_000001.10 NT_167186.1 NG_011566.1
Ensembl:   ENSG00000186197
Entrez:   128178
Chromosome Mapping:   1q42.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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