PrimePCR™ Probe Assay: RGS7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039607
Assay Design:   Intron-spanning
Chromosome Location:   1:240990404-241031966question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000440928 ENST00000348120 ENST00000366563 ENST00000366564 ENST00000366565 ENST00000366562 ENST00000407727 ENST00000401882 ENST00000331110 ENST00000446183

Gene Information

Description Not Available

Gene Symbol:   RGS7
Gene Name:   regulator of G-protein signaling 7
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000182901
Entrez:   6000
UniGene:   Hs.655739
Chromosome Mapping:   1q43,1q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.900000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download