This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PDCD6IP, Human
PrimePCR™ Template for Probe Assay: PDCD6IP, Human
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis in intralumenal endosomal vesicle formation and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition the product of this gene binds to the product of the PDCD6 gene a protein required for apoptosis in a calcium-dependent manner. This gene product also binds to endophilins proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq Jan 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.