This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TNIK, Human
PrimePCR™ Template for Probe Assay: TNIK, Human
Germinal center kinases (GCKs) such as TNIK are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al. 1999 [PubMed 10521462]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.