PrimePCR™ Probe Assay: SPEF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039371
Assay Design:   Intron-spanning
Chromosome Location:   5:35628615-35641604question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000282469 ENST00000509059 ENST00000356031 ENST00000510777 ENST00000440995

Gene Information

Description Not Available

Gene Symbol:   SPEF2
Gene Name:   sperm flagellar 2
Aliases:   CT122, FLJ23164, FLJ23577, FLJ25395, KIAA1770, KPL2, MGC102842
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000152582
Entrez:   79925
UniGene:   Hs.298863
Chromosome Mapping:   5p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.440000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download