PrimePCR™ Probe Assay: PHF19, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039183
Assay Design:   Intron-spanning
Chromosome Location:   9:123632776-123636334question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000373896 ENST00000436309 ENST00000312189 ENST00000456291

Gene Information

Description Not Available

Gene Symbol:   PHF19
Gene Name:   PHD finger protein 19
Aliases:   MGC131698, MGC149712, MGC149713, MGC23929, MTF2L1, PCL3
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000119403
Entrez:   26147
UniGene:   Hs.460124
Chromosome Mapping:   9q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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