PrimePCR™ Probe Assay: MAD2L2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039164
Assay Design:   Intron-spanning
Chromosome Location:   1:11737629-11740464question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000235310 ENST00000376672 ENST00000376669 ENST00000376667 ENST00000445656 ENST00000456915 ENST00000376692

Gene Information

The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein which is similar to MAD2L1 is capable of interacting with ADAM9 ADAM15 REV1 and REV3 proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   MAD2L2
Gene Name:   MAD2 mitotic arrest deficient-like 2 (yeast)
Aliases:   MAD2B, REV7
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000116670
Entrez:   10459
UniGene:   Hs.19400
Chromosome Mapping:   1p36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.950000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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