PrimePCR™ Probe Assay: CCNY, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039129
Assay Design:   Intron-spanning
Chromosome Location:   10:35772348-35805505question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000490012 ENST00000374706 ENST00000493157 ENST00000374704 ENST00000265375

Gene Information

Cyclins such as CCNY control cell division cycles and regulate cyclin-dependent kinases (e.g. CDC2; MIM 116940) (Li et al. 2009 [PubMed 18060517]).[supplied by OMIM May 2009]

Gene Symbol:   CCNY
Gene Name:   cyclin Y
Aliases:   C10orf9, CBCP1, CCNX, CFP1, FLJ95513
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000108100
Entrez:   219771
UniGene:   Hs.14745
Chromosome Mapping:   10p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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