This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SLC2A2, Human
PrimePCR™ Template for Probe Assay: SLC2A2, Human
Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver islet beta cells intestine and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose it has been suggested as a glucose sensor. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.