PrimePCR™ Probe Assay: UBAP2L, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033764
Assay Design:   Intron-spanning
Chromosome Location:   1:154201139-154207167question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000343815 ENST00000441890 ENST00000412596 ENST00000368504 ENST00000437652 ENST00000456325 ENST00000361546 ENST00000271877 ENST00000428931 ENST00000456955 ENST00000433006

Gene Information

Description Not Available

Gene Symbol:   UBAP2L
Gene Name:   ubiquitin associated protein 2-like
Aliases:   FLJ42300, KIAA0144, NICE-4
RefSeq:   NC_000001.10 NT_004487.19 NG_007369.1
Ensembl:   ENSG00000143569
Entrez:   9898
UniGene:   Hs.490551
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.120000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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