This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: RHD, Human
PrimePCR™ Template for Probe Assay: RHD, Human
The Rh blood group system is the second most clinically significant of the blood groups second only to ABO. It is also the most polymorphic of the blood groups with variations due to deletions gene conversions and missense mutations. The Rh blood group includes this gene which encodes the RhD protein and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes and a third unrelated gene are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.