PrimePCR™ Probe Assay: RHD, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033563
Assay Design:   Intron-spanning
Chromosome Location:   1:25611212-25617199question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000328664 ENST00000419831 ENST00000342055 ENST00000357542 ENST00000417538 ENST00000454452 ENST00000423810

Gene Information

The Rh blood group system is the second most clinically significant of the blood groups second only to ABO. It is also the most polymorphic of the blood groups with variations due to deletions gene conversions and missense mutations. The Rh blood group includes this gene which encodes the RhD protein and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes and a third unrelated gene are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RHD
Gene Name:   Rh blood group, D antigen
Aliases:   CD240D, DIIIc, MGC165007, RH, RH30, RHCED, RHDVA(TT), RHDel, RHPII, RHXIII, Rh4, RhDCw, RhII, RhK562-II, RhPI
RefSeq:   NC_000001.10 NG_003255.2 NT_004610.19 NG_007494.1
Ensembl:   ENSG00000187010
Entrez:   6007
UniGene:   Hs.449968
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.010000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download