PrimePCR™ Probe Assay: ABLIM2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033458
Assay Design:   Intron-spanning
Chromosome Location:   4:8046929-8062734question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000361737 ENST00000514025 ENST00000447017 ENST00000341937 ENST00000361581 ENST00000407564 ENST00000505872 ENST00000428004 ENST00000510277 ENST00000400045 ENST00000296372 ENST00000545242 ENST00000546334 ENST00000318888

Gene Information

Description Not Available

Gene Symbol:   ABLIM2
Gene Name:   actin binding LIM protein family, member 2
Aliases:   DKFZp761F129, FLJ26534, FLJ39684, KIAA1808, MGC141918
RefSeq:   NC_000004.11 NT_006051.18
Ensembl:   ENSG00000163995
Entrez:   84448
UniGene:   Hs.233404
Chromosome Mapping:   4p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.110000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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