PrimePCR™ Probe Assay: KRT85, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033394
Assay Design:   Intron-spanning
Chromosome Location:   12:52757151-52757917question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000257901 ENST00000544265

Gene Information

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily consisting of KRTHB1 KRTHB3 and KRTHB6 is highly related. The other less-related subfamily includes KRTHB2 KRTHB4 and KRTHB5. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT85
Gene Name:   keratin 85
Aliases:   HB5, Hb-5, KRTHB5, hHb5
RefSeq:   NC_000012.11 NG_008353.1 NT_029419.12
Ensembl:   ENSG00000135443
Entrez:   3891
UniGene:   Hs.182507
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.640000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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