PrimePCR™ Probe Assay: MOCS1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033358
Assay Design:   Intron-spanning
Chromosome Location:   6:39881535-39883859question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000373186 ENST00000373188 ENST00000373195 ENST00000340692 ENST00000425303 ENST00000432280 ENST00000341481 ENST00000308559 ENST00000373175

Gene Information

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA whereas additional splice variants whose full-length natures have yet to be determined are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq Jan 2010]

Gene Symbol:   MOCS1
Gene Name:   molybdenum cofactor synthesis 1
Aliases:   KIAA0381, MIG11, MOCOD
RefSeq:   NC_000006.11 NG_009297.1 NT_007592.15
Ensembl:   ENSG00000124615
Entrez:   4337
UniGene:   Hs.718492
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.000000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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