PrimePCR™ Probe Assay: MXI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MXI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MXI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033353
Assay Design:   Intron-spanning
Chromosome Location:   10:112039760-112044662question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000332674 ENST00000361248 ENST00000239007 ENST00000393134 ENST00000369614 ENST00000369613 ENST00000369612 ENST00000369619

Gene Information

Expression of the c-myc gene which produces an oncogenic transcription factor is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq Jul 2008]

Gene Symbol:   MXI1
Gene Name:   MAX interactor 1
Aliases:   MAD2, MGC43220, MXD2, MXI, bHLHc11
RefSeq:   NC_000010.10 NT_030059.13 NG_012103.1
Ensembl:   ENSG00000119950
Entrez:   4601
Chromosome Mapping:   10q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 33.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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