PrimePCR™ Probe Assay: IL21R, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033317
Assay Design:   Intron-spanning
Chromosome Location:   16:27448929-27454335question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000337929 ENST00000395755 ENST00000395754

Gene Information

The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors and has been shown to form a heterodimeric receptor complex with the common gamma-chain a receptor subunit also shared by the receptors for interleukin 2 4 7 9 and 15. This receptor transduces the growth promoting signal of IL21 and is important for the proliferation and differentiation of T cells B cells and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules including JAK1 JAK3 STAT1 and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2010]

Gene Symbol:   IL21R
Gene Name:   interleukin 21 receptor
Aliases:   MGC10967, NILR
RefSeq:   NC_000016.9 NT_010393.16 NG_012222.1
Ensembl:   ENSG00000103522
Entrez:   50615
UniGene:   Hs.210546
Chromosome Mapping:   16p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download