This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: IL21R, Human
PrimePCR™ Template for Probe Assay: IL21R, Human
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors and has been shown to form a heterodimeric receptor complex with the common gamma-chain a receptor subunit also shared by the receptors for interleukin 2 4 7 9 and 15. This receptor transduces the growth promoting signal of IL21 and is important for the proliferation and differentiation of T cells B cells and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules including JAK1 JAK3 STAT1 and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.