PrimePCR™ Probe Assay: PPP1CC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPP1CC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP1CC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033171
Assay Design:   Intron-spanning
Chromosome Location:   12:111160417-111162493question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000335007 ENST00000340766 ENST00000551582 ENST00000553024 ENST00000546933 ENST00000550991 ENST00000551676

Gene Information

The protein encoded by this gene belongs to the protein phosphatase family PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes including cell division. It is expressed in mammalian cells as three closely related isoforms alpha beta/delta and gamma which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   PPP1CC
Gene Name:   protein phosphatase 1, catalytic subunit, gamma isozyme
Aliases:   PP-1G, PPP1G
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000186298
Entrez:   5501
Chromosome Mapping:   12q24.1-q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.270000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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