This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: EVC2, Human
PrimePCR™ Template for Probe Assay: EVC2, Human
This gene encodes a protein that functions in bone formation and skeletal development. Mutations in this gene as well as in a neighboring gene that lies in a head-to-head configuration cause Ellis-van Creveld syndrome an autosomal recessive skeletal dysplasia that is also known as chondroectodermal dysplasia. Mutations in this gene also cause acrofacial dysostosis Weyers type also referred to as Curry-Hall syndrome a disease that combines limb and facial abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.