This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: METAP1D, Human
PrimePCR™ Template for Probe Assay: METAP1D, Human
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins thus facilitating subsequent protein modification. In mitochondria enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps or MAPs; EC 188.8.131.52) (Serero et al. 2003 [PubMed 14532271]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.