PrimePCR™ Probe Assay: PAFAH1B2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PAFAH1B2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PAFAH1B2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033099
Assay Design:   Intron-spanning
Chromosome Location:   11:117031920-117034587question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000527958 ENST00000529887 ENST00000530272 ENST00000419197

Gene Information

Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [providedby RefSeq May 2010]

Gene Symbol:   PAFAH1B2
Gene Name:   platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
Aliases:   Not Available
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000168092
Entrez:   5049
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.400000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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