PrimePCR™ Probe Assay: IL1RL2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032927
Assay Design:   Intron-spanning
Chromosome Location:   2:102849504-102851408question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000264257 ENST00000441515 ENST00000539491

Gene Information

The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes including interleukin 1 receptor type I (IL1R1) interleukin 1 receptor type II (IL1R2) interleukin 1 receptor-like 1 (IL1RL1) and interleukin 18 receptor 1 (IL18R1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq Jul 2008]

Gene Symbol:   IL1RL2
Gene Name:   interleukin 1 receptor-like 2
Aliases:   IL1R-rp2, IL1RRP2
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115598
Entrez:   8808
UniGene:   Hs.659863
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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