PrimePCR™ Probe Assay: DLD, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032854
Assay Design:   Intron-spanning
Chromosome Location:   7:107546721-107556006question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000205402 ENST00000417551 ENST00000440410 ENST00000437604 ENST00000539590 ENST00000537148

Gene Information

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein also named dihydrolipoamide dehydrogenase is also a component of the pyruvate dehydrogenase complex the alpha-ketoglutarate dehydrogenase complex and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:   DLD
Gene Name:   dihydrolipoamide dehydrogenase
Aliases:   DLDH, E3, GCSL, LAD, PHE3
RefSeq:   NC_000007.13 NG_008045.1 NT_007933.15
Ensembl:   ENSG00000091140
Entrez:   1738
UniGene:   Hs.131711
Chromosome Mapping:   7q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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