This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SMG6, Human
PrimePCR™ Template for Probe Assay: SMG6, Human
This gene encodes a protein which is part of the telomerase ribonucleoprotein complex and binds single-stranded DNA found at the telomeres but the product of this gene also participates in the nonsense-mediated mRNA decay (NMD) pathway. The encoded protein contains two binding sites for components of the exon junction complex (EJC); interaction between the encoded protein and the EJC is necessary for NMD activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.