PrimePCR™ Probe Assay: PLEKHA5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032829
Assay Design:   Intron-spanning
Chromosome Location:   12:19406889-19408042question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000429027 ENST00000299275 ENST00000538714 ENST00000538305 ENST00000536974 ENST00000317589 ENST00000355397 ENST00000359180 ENST00000542828 ENST00000309364 ENST00000412219 ENST00000424268 ENST00000543806 ENST00000539256

Gene Information

Description Not Available

Gene Symbol:   PLEKHA5
Gene Name:   pleckstrin homology domain containing, family A member 5
Aliases:   FLJ10667, FLJ26734, FLJ31492, KIAA1686, PEPP-2, PEPP2
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000052126
Entrez:   54477
UniGene:   Hs.188614
Chromosome Mapping:   12p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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