PrimePCR™ Probe Assay: PDSS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDSS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDSS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031955
Assay Design:   Intron-spanning
Chromosome Location:   10:27031447-27035361question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000376215 ENST00000376203 ENST00000396343

Gene Information

The protein encoded by this gene is an enzyme that elongates the prenyl side-chain of coenzyme Q or ubiquinone one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains the first step in coenzyme Q biosynthesis. The protein may be peripherally associated with the inner mitochondrial membrane though no transit peptide has been definitively identified to date. Defects in this gene are a cause of coenzyme Q10 deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:   PDSS1
Gene Name:   prenyl (decaprenyl) diphosphate synthase, subunit 1
Aliases:   COQ1, DPS, MGC70953, RP13-16H11.3, SPS, TPRT, TPT, TPT 1, hDPS1
RefSeq:   NC_000010.10 NG_008972.1 NT_008705.16
Ensembl:   ENSG00000148459
Entrez:   23590
Chromosome Mapping:   10p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.460000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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