This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CSE1L, Human
PrimePCR™ Template for Probe Assay: CSE1L, Human
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS while importin-beta mediates translocation through the nuclear pore complex. After translocation RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jan 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.