PrimePCR™ Probe Assay: AASDH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AASDH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AASDH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031921
Assay Design:   Intron-spanning
Chromosome Location:   4:57248691-57250293question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000205214 ENST00000502617 ENST00000434343 ENST00000451613

Gene Information

Description Not Available

Gene Symbol:   AASDH
Gene Name:   aminoadipate-semialdehyde dehydrogenase
Aliases:   ACSF4, LYS2, NRPS1098, NRPS998
RefSeq:   NC_000004.11 NT_022853.15
Ensembl:   ENSG00000157426
Entrez:   132949
Chromosome Mapping:   4q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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