PrimePCR™ Probe Assay: PLAA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031901
Assay Design:   Intron-spanning
Chromosome Location:   9:26910354-26917097question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000397292 ENST00000517642 ENST00000487173 ENST00000520884

Gene Information

Description Not Available

Gene Symbol:   PLAA
Gene Name:   phospholipase A2-activating protein
Aliases:   DOA1, FLJ11281, FLJ12699, PLA2P, PLAP
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000137055
Entrez:   9373
UniGene:   Hs.27182
Chromosome Mapping:   9p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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