PrimePCR™ Probe Assay: GLRA3, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031891
Assay Design:   Intron-spanning
Chromosome Location:   4:175580264-175598301question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000274093 ENST00000340217

Gene Information

The GLRA3 gene encodes the alpha-3 subunit of the neuronal glycine receptor a ligand-gated chloride channel composed of ligand-binding alpha and structural beta polypeptides (Kingsmore et al. 1994 [PubMed 7894176]).[supplied by OMIM Nov 2009]

Gene Symbol:   GLRA3
Gene Name:   glycine receptor, alpha 3
Aliases:   Not Available
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000145451
Entrez:   8001
UniGene:   Hs.413099
Chromosome Mapping:   4q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 33.590000
Efficiency 110

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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