This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: COPA, Human
PrimePCR™ Template for Probe Assay: COPA, Human
In eukaryotic cells protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP beta-COP beta-prime-COP gamma-COP delta-COP epsilon-COP and zeta-COP. The alpha-COP encoded by COPA shares high sequence similarity with RET1P the alpha subunit of the coatomer complex in yeast. Also the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide xenin which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.