PrimePCR™ Probe Assay: CNTNAP2, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031870
Assay Design:   Intron-spanning
Chromosome Location:   7:148106523-148112585question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000361727 ENST00000538075

Gene Information

This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein like other neurexin proteins contains epidermal growth factor repeats and laminin G domains. In addition it includes an F5/8 type C domain discoidin/neuropilin- and fibrinogen-like domains thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2 (FOXP2) a transcription factor related to speech and language development. This gene has been implicated in multiple neurodevelopmental disorders including Gilles de la Tourette syndrome schizophrenia epilepsy autism ADHD and mental retardation.[provided by RefSeq Mar 2010]

Gene Symbol:   CNTNAP2
Gene Name:   contactin associated protein-like 2
Aliases:   AUTS15, CASPR2, CDFE, DKFZp781D1846, NRXN4, PTHSL1
RefSeq:   NC_000007.13 NG_007092.2 NT_007914.15
Ensembl:   ENSG00000174469
Entrez:   26047
UniGene:   Hs.655684
Chromosome Mapping:   7q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.250000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download