PrimePCR™ Probe Assay: PHYH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031849
Assay Design:   Intron-spanning
Chromosome Location:   10:13333858-13336518question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000396913 ENST00000263038 ENST00000396920 ENST00000453759 ENST00000479604

Gene Information

This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   PHYH
Gene Name:   phytanoyl-CoA 2-hydroxylase
Aliases:   LN1, LNAP1, PAHX, PHYH1, RD
RefSeq:   NC_000010.10 NT_008705.16 NG_012862.1
Ensembl:   ENSG00000107537
Entrez:   5264
UniGene:   Hs.498732
Chromosome Mapping:   10p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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