PrimePCR™ Probe Assay: BAI1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031816
Assay Design:   Intron-spanning
Chromosome Location:   8:143561062-143562700question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000517894 ENST00000323289

Gene Information

Angiogenesis is controlled by a local balance between stimulators and inhibitors of new vessel growth and is suppressed under normal physiologic conditions. Angiogenesis has been shown to be essential for growth and metastasis of solid tumors. In order to obtain blood supply for their growth tumor cells are potently angiogenic and attract new vessels as results of increased secretion of inducers and decreased production of endogenous negative regulators. BAI1 contains at least one 'functional' p53-binding site within an intron and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures however only BAI1 is transcriptionally regulated by p53. BAI1 is postulated to be a member of the secretin receptor family an inhibitor of angiogenesis and a growth suppressor of glioblastomas [provided by RefSeq Jul 2008]

Gene Symbol:   BAI1
Gene Name:   brain-specific angiogenesis inhibitor 1
Aliases:   FLJ41988, GDAIF
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000181790
Entrez:   575
UniGene:   Hs.194654
Chromosome Mapping:   8q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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