PrimePCR™ Probe Assay: IGF2R, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031773
Assay Design:   Intron-spanning
Chromosome Location:   6:160517596-160523630question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000356956

Gene Information

The protein encoded by this gene is a receptor for both insulin-like growth factor 2 (IGF2) and mannose 6-phosphate (M6P). The IGF2 and M6P binding sites are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes the activation of transforming growth factor beta and the degradation of IGF2. While the mouse Igf2r gene shows exclusive expression from the maternal allele imprinting of the human IGF2R gene appears to be polymorphic with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq Oct 2010]

Gene Symbol:   IGF2R
Gene Name:   insulin-like growth factor 2 receptor
Aliases:   CD222, CIMPR, M6P-R, MPR1, MPRI
RefSeq:   NC_000006.11 NG_011785.1 NT_025741.15
Ensembl:   ENSG00000197081
Entrez:   3482
UniGene:   Hs.487062
Chromosome Mapping:   6q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.140000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download