This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PFKFB2, Human
PrimePCR™ Template for Probe Assay: PFKFB2, Human
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-26-bisphosphate a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-26-bisphosphate and a fructose-26-biphosphatase activity that catalyzes the degradation of fructose-26-bisphosphate. This protein regulates fructose-26-bisphosphate levels in the heart while a related enzyme encoded by a different gene regulates fructose-26-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.