PrimePCR™ Probe Assay: CAPN2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031689
Assay Design:   Intron-spanning
Chromosome Location:   1:223949889-223954097question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000295006 ENST00000433674 ENST00000366869

Gene Information

The calpains calcium-activated neutral proteases are nonlysosomal intracellular cysteine proteases. The mammalian calpains include ubiquitous stomach-specific and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large catalytic subunits associated with a common small regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   CAPN2
Gene Name:   calpain 2, (m/II) large subunit
Aliases:   CANP2, CANPL2, CANPml, FLJ39928, mCANP
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162909
Entrez:   824
UniGene:   Hs.350899
Chromosome Mapping:   1q41-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.110000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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