This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CAPN2, Human
PrimePCR™ Template for Probe Assay: CAPN2, Human
The calpains calcium-activated neutral proteases are nonlysosomal intracellular cysteine proteases. The mammalian calpains include ubiquitous stomach-specific and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large catalytic subunits associated with a common small regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.