This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DOCK2, Human
PrimePCR™ Template for Probe Assay: DOCK2, Human
The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells predominantly in the peripheral blood leukocytes and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.