PrimePCR™ Probe Assay: DOCK2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031654
Assay Design:   Intron-spanning
Chromosome Location:   5:169230124-169267794question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000256935 ENST00000520908 ENST00000519628 ENST00000343291 ENST00000540750

Gene Information

The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells predominantly in the peripheral blood leukocytes and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq Oct 2011]

Gene Symbol:   DOCK2
Gene Name:   dedicator of cytokinesis 2
Aliases:   FLJ46592, KIAA0209
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000134516
Entrez:   1794
UniGene:   Hs.586174
Chromosome Mapping:   5q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 33.870000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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