PrimePCR™ Probe Assay: MYO5A, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031644
Assay Design:   Intron-spanning
Chromosome Location:   15:52681459-52684175question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000399231 ENST00000399229 ENST00000553916 ENST00000399233 ENST00000356338 ENST00000358212 ENST00000546028

Gene Information

This gene is one of three myosin V heavy-chain genes belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1) Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported but the full-length nature of some variants has not been determined. [provided by RefSeq Dec 2008]

Gene Symbol:   MYO5A
Gene Name:   myosin VA (heavy chain 12, myoxin)
Aliases:   GS1, MYH12, MYO5, MYR12
RefSeq:   NC_000015.9 NG_009887.1 NT_010194.17
Ensembl:   ENSG00000197535
Entrez:   4644
UniGene:   Hs.21213
Chromosome Mapping:   15q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download