This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ATP11B, Human
PrimePCR™ Template for Probe Assay: ATP11B, Human
P-type ATPases such as ATP11B are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions such as H(+) Na(+) K(+) or Ca(+). A third subfamily transports amphipaths such as phosphatidylserine.[supplied by OMIM Feb 2005]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.