PrimePCR™ Probe Assay: PIWIL4, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031619
Assay Design:   Intron-spanning
Chromosome Location:   11:94310558-94316650question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000299001 ENST00000545603

Gene Information

PIWIL4 belongs to the Argonaute family of proteins which function in development and maintenance of germline stem cells (Sasaki et al. 2003 [PubMed 12906857]).[supplied by OMIM Mar 2008]

Gene Symbol:   PIWIL4
Gene Name:   piwi-like 4 (Drosophila)
Aliases:   DKFZp686P01248, FLJ36156, HIWI2, MIWI2
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000134627
Entrez:   143689
UniGene:   Hs.660188
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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