PrimePCR™ Probe Assay: HIVEP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HIVEP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIVEP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031606
Assay Design:   Intron-spanning
Chromosome Location:   6:143075027-143080985question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000367604 ENST00000012134 ENST00000367603

Gene Information

Members of the ZAS family such as ZAS2 (HIVEP2) are large proteins that contain a ZAS domain a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. These proteins bind specific DNA sequences including the kappa-B motif (GGGACTTTCC) in the promoters and enhancer regions of several genes and viruses including human immunodeficiency virus (HIV). ZAS genes span more than 150 kb and contain at least 10 exons one of which is longer than 5.5 kb (Allen and Wu 2004).[supplied by OMIM Apr 2006]

Gene Symbol:   HIVEP2
Gene Name:   human immunodeficiency virus type I enhancer binding protein 2
Aliases:   HIV-EP2, MBP-2, MIBP1, SHN2, ZAS2, ZNF40B
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000010818
Entrez:   3097
Chromosome Mapping:   6q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.760000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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