This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ING5, Human
PrimePCR™ Template for Probe Assay: ING5, Human
The protein encoded by this gene is similar to ING1 a tumor suppressor protein that can interact with TP53 inhibit cell growth and induce apoptosis. This protein contains a PHD-finger which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300 a component of the histone acetyl transferase complex suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.