PrimePCR™ Probe Assay: CALB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CALB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CALB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031558
Assay Design:   Intron-spanning
Chromosome Location:   16:71411570-71416655question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000302628 ENST00000349553

Gene Information

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability and is a diagnostic marker for some human diseases including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jun 2010]

Gene Symbol:   CALB2
Gene Name:   calbindin 2
Aliases:   CAB29, CAL2, CR
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000172137
Entrez:   794
Chromosome Mapping:   16q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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