PrimePCR™ Probe Assay: AXIN2, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031547
Assay Design:   Intron-spanning
Chromosome Location:   17:63526141-63530076question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000307078 ENST00000375702

Gene Information

The Axin-related protein Axin2 presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway like its rodent homologs mouse conductin/rat axil. In mouse conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon) beta-catenin glycogen synthase kinase 3-beta and conductin which leads to the degradation of beta-catenin. Apparently the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24 a region that shows frequent loss of heterozygosity in breast cancer neuroblastoma and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq Jul 2008]

Gene Symbol:   AXIN2
Gene Name:   axin 2
Aliases:   AXIL, DKFZp781B0869, MGC10366, MGC126582
RefSeq:   NC_000017.10 NG_012142.1 NT_010783.15
Ensembl:   ENSG00000168646
Entrez:   8313
UniGene:   Hs.156527
Chromosome Mapping:   17q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.520000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.