PrimePCR™ Probe Assay: DNAH5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031524
Assay Design:   Intron-spanning
Chromosome Location:   5:13769636-13770947question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000265104

Gene Information

This gene encodes a dynein protein which is part of a microtubule-associated motor protein complex consisting of heavy light and intermediate chains. This protein is an axonemal heavy chain dynein. It functions as a force-generating protein with ATPase activity whereby the release of ADP is thought to produce the force-producing power stroke. Mutations in this gene cause primary ciliary dyskinesia type 3 as well as Kartagener syndrome which are both diseases due to ciliary defects. [provided by RefSeq Oct 2009]

Gene Symbol:   DNAH5
Gene Name:   dynein, axonemal, heavy chain 5
Aliases:   CILD3, DNAHC5, FLJ46759, HL1, KIAA1603, KTGNR, PCD
RefSeq:   NC_000005.9 NG_013081.1 NT_006576.16
Ensembl:   ENSG00000039139
Entrez:   1767
UniGene:   Hs.212360
Chromosome Mapping:   5p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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