PrimePCR™ Probe Assay: PIWIL1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031498
Assay Design:   Intron-spanning
Chromosome Location:   12:130831547-130833816question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000245255 ENST00000540672

Gene Information

This gene encodes a member of the PIWI subfamily of Argonaute proteins evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal RNA silencing and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   PIWIL1
Gene Name:   piwi-like 1 (Drosophila)
Aliases:   HIWI, MIWI, PIWI
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000125207
Entrez:   9271
UniGene:   Hs.405659
Chromosome Mapping:   12q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.991000
y-intercept 33.330000
Efficiency 110

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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