PrimePCR™ Probe Assay: FANCA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FANCA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FANCA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031497
Assay Design:   Intron-spanning
Chromosome Location:   16:89880938-89882379question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000389301 ENST00000534992 ENST00000389302 ENST00000543736

Gene Information

The Fanconi anemia complementation group (FANC) currently includes FANCA FANCB FANCC FANCD1 (also called BRCA2) FANCD2 FANCE FANCF FANCG FANCI FANCJ (also called BRIP1) FANCL FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability hypersensitivity to DNA crosslinking agents increased chromosomal breakage and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia. [provided by RefSeq Jul 2008]

Gene Symbol:   FANCA
Gene Name:   Fanconi anemia, complementation group A
Aliases:   FA, FA-H, FA1, FAA, FACA, FAH, FANCH, MGC75158
RefSeq:   NC_000016.9 NG_011706.1 NT_010542.15
Ensembl:   ENSG00000187741
Entrez:   2175
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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