PrimePCR™ Probe Assay: DNAH10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DNAH10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DNAH10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031482
Assay Design:   Intron-spanning
Chromosome Location:   12:124293361-124297733question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000409039

Gene Information

Dyneins are microtubule-associated motor protein complexes composed of several heavy light and intermediate chains. The axonemal dyneins found in cilia and flagella are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAH10 is an inner arm dynein heavy chain (Maiti et al. 2000 [PubMed 11175280]).[supplied by OMIM Mar 2008]

Gene Symbol:   DNAH10
Gene Name:   dynein, axonemal, heavy chain 10
Aliases:   FLJ38262, FLJ43486, FLJ43808, KIAA2017
RefSeq:   NG_012743.1 NC_000012.11 NT_009755.19
Ensembl:   ENSG00000197653
Entrez:   196385
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.280000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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