PrimePCR™ Probe Assay: SUMF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SUMF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SUMF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031465
Assay Design:   Intron-spanning
Chromosome Location:   3:4494654-4508690question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000272902 ENST00000383843 ENST00000458465 ENST00000405420 ENST00000534982 ENST00000534863

Gene Information

This gene encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq Sep 2009]

Gene Symbol:   SUMF1
Gene Name:   sulfatase modifying factor 1
Aliases:   AAPA3037, FGE, MGC131853, MGC150436
RefSeq:   NC_000003.11 NT_022517.18 NG_016225.1
Ensembl:   ENSG00000144455
Entrez:   285362
Chromosome Mapping:   3p26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.210000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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