This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: XYLB, Human
PrimePCR™ Template for Probe Assay: XYLB, Human
The protein encoded by this gene shares 22% sequence identity with Hemophilus influenzae xylulokinase and even higher identity to other gene products in C.elegans (45%) and yeast (31-35%) which are thought to belong to a family of enzymes that include fucokinase gluconokinase glycerokinase and xylulokinase. These proteins play important roles in energy metabolism. [provided by RefSeq Aug 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.