PrimePCR™ Probe Assay: XYLB, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031458
Assay Design:   Intron-spanning
Chromosome Location:   3:38409687-38411649question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000207870 ENST00000542835

Gene Information

The protein encoded by this gene shares 22% sequence identity with Hemophilus influenzae xylulokinase and even higher identity to other gene products in C.elegans (45%) and yeast (31-35%) which are thought to belong to a family of enzymes that include fucokinase gluconokinase glycerokinase and xylulokinase. These proteins play important roles in energy metabolism. [provided by RefSeq Aug 2009]

Gene Symbol:   XYLB
Gene Name:   xylulokinase homolog (H. influenzae)
Aliases:   FLJ10343, FLJ12539, FLJ22075
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000093217
Entrez:   9942
UniGene:   Hs.659985
Chromosome Mapping:   3p22-p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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